PNGase F from Promega

Product Specs
ItemPNGase F
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV4831
Quantity500 units

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Identification of tyrosine sulfation in the variable region of a bispecific antibody and its effect on stability and biological activitymAbsDecember 31, 2023Christopher B. Lietz, Ekaterina Deyanova, Younhee Cho, Jon Cordia, Sarah Franc, Sally Kabro, Steven Wang, David Mikolon, Douglas D. Banksby incubating 1 mg/mL of mAb-X buffered by 50 mM Tris-HCl (pH 7.5) with PNGase F (Promega, Catalog # V4831) at a final enzyme concentration of 1 U/µL. Samples were incubated overnight at 37°C prior to intact

(2) CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering processCancer Gene TherapyOctober 1, 2023Bianca Altvater, Sareetha Kailayangiri, Christian Spurny, Maike Flügge, Jutta Meltzer, Lea Greune, Katja Urban, Christian Schwöppe, Caroline Brand, Christoph Schliemann, et al.ice 2 µl GlycoBuffer 2 (10X), 2 µl 10% NP-40, 1 µl PNGase F (Promega, Walldorf, Germany, product no. V4831) and 6 µl H2O were added, mixed and incubated at 37 °C for 1 h. Factor X (FX) activation assay The

(3) Analyzing the topology of N-linked glycans by PNGase F accessibility assaySTAR ProtocolsSeptember 1, 2023Jingcheng Wang, Jin Yeproteins" ,, Pierce Protease Inhibitor (Cocktail) Mini Tablets,Thermo Fisher,A32955 PNGase F,Promega,V4831 "0.5 M Tris-HCl, pH 6.8",Bio-Rad,1610799 (PBS) Dulbecco’s Phosphate-Buffered Saline without calcium

(4) Development of an Affinity-Based Probe to Profile Endogenous Human Adenosine A3 Receptor ExpressionJournal of Medicinal ChemistryAugust 24, 2023Bert L. H. Beerkens, Inge M. Snijders, Joep Snoeck, Rongfang Liu, Anton T. J. Tool, Sylvia E. Le Dévédec, Willem Jespers, Taco W. Kuijpers, Gerard J.P. van Westen, Laura H. Heitman, et al.pH 7.4). Eosinophils were isolated from the PMNs as described before.68 Biologicals PNGase (cat# V4831) was purchased from Promega (Leiden, The Netherlands). RabbitαhA3AR antibody (cat# bs-1225R) was ordered

(5) Multi-Attribute Monitoring Method for Process Development of Engineered Antibody for Site-Specific ConjugationJournal of the American Society for Mass SpectrometryJuly 5, 2023Alistair R. Hines, Matthew Edgeworth, Paul W. A. Devine, Samuel Shepherd, Nicholas Chatterton, Claire Turner, Kathryn S. Lilley, Xiaoyu Chen, Nicholas J. Bondconducted through UPLC analysis. Antibody intermediate (100 mg) was digested overnight with PNGaseF (V4831, Promega) at 37 °C, and subsequently labeled with 2-aminobenzamide (PN 654213, Sigma-Aldrich) for 30

(6) Comprehensive glycoprofiling of oral tumours associates N-glycosylation with lymph node metastasis and patient survivalMolecular & Cellular ProteomicsJuly 1, 2023Carolina Moretto Carnielli, Thayná Melo de Lima Morais, Fábio Malta de Sá Patroni, Ana Carolina Prado Ribeiro, Thaís Bianca Brandão, Evandro Sobroza, Leandro Luongo Matos, Luiz Paulo Kowalski, Adriana Franco Paes Leme, Rebeca Kawahara, et al.N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μl) for 16 h at 37 °C. The detached N-glycans were reduced with 1 M sodium borohydride in 50 mM

(7) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha lytic protease,New England BioLabs,Cat#P8113 18O

(8) Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by OmicroniScienceApril 21, 2023Jakob Kreye, S. Momsen Reincke, Stefan Edelburg, Lara M. Jeworowski, Hans-Christian Kornau, Jakob Trimpert, Peter Hombach, Sophia Halbe, Volker Nölle, Martin Meyer, et al.MabSelectTM,Cytiva,Cat# 28408256 MACSQuant® Tyto® Cartridges,Miltenyi Biotec,Cat# 130-104-791 PNGase F,Promega,Cat# V4831 OCT medium (Roth).,Roth,Cat# 6478.1 Ventana diluent,Ventana,Cat# 760-108 DAB Map detection system,Ventana,Cat#

(9) Topological regulation of a transmembrane protein by luminal-to-cytosolic retrotranslocation of glycosylated sequenceCell ReportsApril 1, 2023Jingcheng Wang, Sungwon Han, Jin Yewere incubated in 50 mM sodium phosphate in the absence or presence of 0.25 U/μl PNGase F (Promega V4831), 0.5% NP-40, and/or 0.5% SDS at 30°C for 45 min. After cooling to 4°C, the reaction was terminated

(10) Cyclophosphamide depletes tumor infiltrating T regulatory cells and combined with anti-PD-1 therapy improves survival in murine neuroblastomaiScienceSeptember 16, 2022Webb ER, Moreno-Vincente J, Easton A, Lanati S, Taylor M, James S, Williams EL, English V, Penfold C, Beers SA, et al.Biosciences,https://www.bdbiosciences.com/en-gb/products/software/instrument-software/bd-facsdiva-software ,, Other,Other,Other ,, PNGase F,Promega,Cat#V4831 Liberase TL,Merck (Roche),Cat#V4831 DNAse,Sigma-Aldrich,Cat#10104159001 100 μm cell strainer,Corning,Cat#352360 Red

(11) An optimized protocol for PD-L1 pathological assessment with patient sample deglycosylation to improve correlation with therapeutic responseSTAR ProtocolsMarch 18, 2022Ying-Nai Wang, Heng-Huan Lee, Yongkun Wei, Yu-Yi Chu, Weiya Xia, Michael Wang, Dihua Yu, Mien-Chie Hung(Cat#P0704). However, it should be also applicable to that from different vendors, such as from Promega (Cat#V4831) or Sigma-Aldrich (Cat#G5166). We have not evaluated it using other PNGase F proteins, but we extrapolate

(12) The Trimeric Autotransporter Adhesin YadA of Yersinia enterocolitica Serotype O:9 Binds Glycan MoietiesFrontiers in MicrobiologyFebruary 1, 2022Ina Meuskens, Juan Leva-Bueno, Paul Millner, Monika Schütz, Sally A. Peyman, Dirk Linkerespective sources (in water) was mixed with 2 μg of the glycopeptidase PNGase F (500 U) (Promega, V4831) and incubated at 37°C for 19 h. The non-deglycosylated control samples of Cn were incubated at 37°C

(13) Quantitative N-glycoproteome analysis of bovine milk and yogurtCurrent Research in Food ScienceJanuary 12, 2022Jing Xiao, Jinqiu Wang, Renyou Gan, Di Wu, Yisha Xu, Lianxin Peng, Fang Gengvia hydrophilic interaction liquid chromatography and deglycosylated with peptide-N-glycosidase F (V4831, Promega Corporation, Madison, WI, USA) in H218O at 37 °C overnight (Xiao et al., 2020). Deglycopeptides

(14) Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyteCardiovascular DiabetologyDecember 1, 2021Nunzia D’Onofrio et al.heterogeneity of hACE2 was removed by overnight incubation at 37 °C with PNGase F (Promega, catalog no. V4831), and then evaluated the result by SDS-PAGE. NIST8671 monoclonal antibody was glycosylated in the same

(15) The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic PainBiomoleculesNovember 12, 2021Rodríguez-Muñoz M, Cortés-Montero E, Onetti Y, Sánchez-Blázquez P, Garzón-Niño J.SDS from the proteins and then incubated for 18 h at 37 °C with PNGase F (5 units/10 μg of protein, #V4831: Promega, Madrid, Spain). The samples were then concentrated, solubilized in Laemmli buffer, separated

(16) Molecular architecture and modifications of full-length myocilinExperimental Eye ResearchOctober 1, 2021Mackenzie D. Martin, Dustin J. E. Huard, Ricardo C. Guerrero-Ferreira, Ishani M. Desai, Brett M. Barlow, Raquel L. Liebermanindividual deglycosylation enzymes from the kit (NEB P0709S, P0733S, P0722S, P0745S, and P0721S or Promega V4831) according to associated NEB and Promega protocols. For reactions conducted under non-denaturing conditions,

(17) Global mapping of glycosylation pathways in human-derived cellsDevelopmental CellApril 1, 2021Yi-Fan Huang et al.sorter,Bio-Rad,S3e T100 Thermal Cycler,Bio-Rad,T100 iMark Microplate Absorbance Reader,Bio-Rad,N/A PNGaseF,Promega,Cat#V4831 "Sep-Pak Vac C18 1cc, 100mg",Waters,WAT023590 Sep-Pak Plus Light tC18,Waters,WAT036805 Supelclean

(18) The Complexity and Dynamics of the Tissue Glycoproteome Associated With Prostate Cancer ProgressionMolecular & Cellular ProteomicsJanuary 5, 2021Rebeca Kawahara, Saulo Recuero, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Giuseppe PalmisanoN-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega; V4831, 10 U/μl), 16 h, and 37 °C. The detached glycans were reduced with 1 M sodium borohydride in 50 mM aqueous

(19) Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike proteinSTAR ProtocolsDecember 30, 2020Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhaoreagent",Sigma-Aldrich,320331 Dithiothreitol,Sigma-Aldrich,43815 Iodoacetamide,Sigma-Aldrich,I1149 Trypsin,Promega,V5111 Lys-C,Promega,V1671 Arg-C,Promega,V1881 Glu-C,Promega,V1651 Asp-N,Promega,VA1160 Endoglycosidase H,Promega,V4871 PNGaseF,Promega,V4831 Chymotrypsin,Athens Research and Technology,16-19-030820 Alpha lytic protease,New England BioLabs,P8113 "Water

(20) Calmodulin Supports TRPA1 Channel Association with Opioid Receptors and Glutamate NMDA Receptors in the Nervous TissueInternational journal of molecular sciencesDecember 28, 2020Cortés-Montero E, Rodríguez-Muñoz M, Ruiz-Cantero MDC, Cobos EJ, Sánchez-Blázquez P, Garzón-Niño J.with 0.65% octylthioglucoside to help remove SDS from the proteins and then incubated with PNGase F (V4831, Promega, Madrid, Spain) for 18 h at 37 °C (5 units/10 μg of protein). The samples were then concentrated,

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PNGase F

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PNGase F from Promega

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